K. Nitta et al., INHIBITION OF CELL-PROLIFERATION BY RANA-CATESBEIANA AND RANA-JAPONICA LECTINS BELONGING TO THE RIBONUCLEASE SUPERFAMILY, Cancer research, 54(4), 1994, pp. 920-927
Two frog egg lectins [Rana catesbeiana lectin (SBL-C) and Rana japonic
a lectin] preferentially agglutinate a large variety of human and anim
al turner cells but not blood cells, lymphocytes, or fibroblasts. Thes
e lectins belong to the superfamily of pyrimidine base-specific RNases
. The two lectins bound to a heparin-Sepharose column and were eluted
from the column by an increase of NaCl molarity. Both their tumor cell
-agglutinating activity and RNase activity were inhibited by heparin,
and also by polyamines, such as spermine. Both lectins inhibited P388
leukemia cell proliferation. The inhibitory activity of SBL-C was bloc
ked by addition of heparin. SBL-C inhibited protein synthesis by P388
cells, but RNase A did not. No lectin-induced antiproliferative effect
was observed after sialidase treatment of cells. The antiproliferativ
e activity of SBL-C was also inhibited by ammonium chloride treatment.
These results suggest that internalization of the lectins by lectin r
eceptor (sialoglycoconjugate)-mediated endocytosis is followed by cell
death due to inhibition of protein synthesis. Administration of SBL-C
i.p. delayed time to death in mice receiving i.p. transplants of Sarc
oma 180 and Mep II cells.