COMPARISON OF DIFFERENT METHODS FOR DETECTION OF LISTERIA-MONOCYTOGENES IN DAIRY-PRODUCTS

Citation
L. Herman et H. Deridder, COMPARISON OF DIFFERENT METHODS FOR DETECTION OF LISTERIA-MONOCYTOGENES IN DAIRY-PRODUCTS, Milchwissenschaft, 48(12), 1993, pp. 684-686
Citations number
9
Categorie Soggetti
Food Science & Tenology
Journal title
ISSN journal
00263788
Volume
48
Issue
12
Year of publication
1993
Pages
684 - 686
Database
ISI
SICI code
0026-3788(1993)48:12<684:CODMFD>2.0.ZU;2-4
Abstract
Three different methods were applied for the detection of Listeria mon ocytogenes in 58 dairy samples. The results obtained with the classica l and the DNA colony hybridization method agreed for all the samples. The PCR detection method in which the enrichment culture was directly tested rendered false negative results for 3 soft cheese samples due t o the presence of components in the enrichment culture of this cheese which inhibits the PCR. On the contrary, the sensitivity of the PCR me thod for cheese wash water was higher than that of the two other metho ds.