ISOLATION AND COMPLETE SEQUENCE OF CBR, A GENE ENCODING A PUTATIVE CYTOCHROME-B REDUCTASE IN SACCHAROMYCES-CEREVISIAE

Authors
CSUKAI M MURRAY M ORR E
Citation
M. Csukai et al., ISOLATION AND COMPLETE SEQUENCE OF CBR, A GENE ENCODING A PUTATIVE CYTOCHROME-B REDUCTASE IN SACCHAROMYCES-CEREVISIAE, European journal of biochemistry, 219(1-2), 1994, pp. 441-448
Citations number
65
Categorie Soggetti
Biology
Journal title
European journal of biochemistry → ACNP
ISSN journal
00142956
Volume
219
Issue
1-2
Year of publication
1994
Pages
441 - 448
Database
ISI
SICI code
0014-2956(1994)219:1-2<441:IACSOC>2.0.ZU;2-Q
Abstract
We have isolated and characterised a novel yeast gene, CBR (cytochrome b reductase), encoding a 35-kDa yeast novobiocin-binding protein. The predicted protein sequence of CBR displays considerable similarity to both plant nitrate reductases and mammalian cytochrome b(5) reductase s indicating that it is a putative member of the flavoprotein pyridine -nucleotide-cytochrome-reductase family. Disruption of CBR is not leth al under various growth conditions, suggesting the presence of some fu nctional overlap with other reductases, possibly with the cytochrome P -450 reductase.