J. Fernandezalvarez et al., ENZYMATIC, METABOLIC AND SECRETORY PATTERNS IN HUMAN ISLETS OF TYPE-2(NON-INSULIN-DEPENDENT) DIABETIC-PATIENTS, Diabetologia, 37(2), 1994, pp. 177-181
Citations number
33
Categorie Soggetti
Endocrynology & Metabolism","Medicine, General & Internal
Islets were isolated by automatic digestion from non-diabetic cadaveri
c organ donors and from Type 2 (non-insulin-dependent) diabetic subjec
ts. The activity of FAD-glycerophosphate dehydrogenase, but not that o
f either glutamate dehydrogenase, glutamate-oxalacetate transaminase o
r glutamate-pyruvate transaminase, was lower in Type 2 diabetic patien
ts than control subjects. Hexokinase, glucokinase and glutamate decarb
oxylase activities were also measured in islets from control subjects.
The utilization of D-[5-H-3]glucose, oxidation of D-[6-C-14]glucose a
nd release of insulin evoked by D-glucose were all lower in Type 2 dia
betic patients than control subjects. The secretory response to the co
mbination of L-leucine and L-glutammine appeared less severely affecte
d. Islets from Type 2 diabetic patients may thus display enzymatic, me
tabolic and secretory anomalies similar to those often observed in ani
mal models of Type 2 diabetes, including a deficiency of beta-cell FAD
-linked glycerophosphate dehydrogenase, the key enzyme of the glycerol
phosphate shuttle.