Ah. Hammond et Jr. Fry, TOXICITY OF PRECOCENE-II IN RAT HEPATOCYTE CULTURES - EFFECTS OF SERUM AND CULTURE TIME, Toxicology letters, 70(3), 1994, pp. 337-342
Omission of Serum from culture medium markedly increased the toxicity
of the cytochrome P450-activated toxin precocene II in 24-h cultures o
f rat hepatocytes. In addition, the depletion of glutathione and inhib
ition of cytochrome P450 in 24-h cultures increased and decreased, res
pectively, the toxicity of precocene II, as seen in vivo. Precocene II
was also toxic to 72-h cultures, although the effect of the serum-fre
e medium was attenuated. However, depletion of glutathione and inhibit
ion of P450 had little or no effect on the toxicity in 72-h cultures.
Therefore, the mechanism of precocene II-induced cell death appears to
change with time in culture.