CTLL ASSAY - COMPARISON OF 2 METHODS FOR IL-2 DETERMINATION

Two different modifications of CTLL techniques for IL-2 detection, the routine H-3-thymidine incorporation assay and the colorimetric MTT me thod, were compared. The reproducible results of the studies of supern atants from in vitro propagated transfected cells were obtained. The e mployed methods appeared to be comparable and are used simultaneously in our co-operating laboratories for estimation of the levels of IL-2 secreted by the transfectants that are applied as the source of the cy tokine in therapeutic experiments.