STATUS OF GLUTATHIONE AND GLUTATHIONE-METABOLIZING ENZYMES IN MENADIONE-RESISTANT HUMAN CANCER-CELLS

Authors
Citation
Eo. Ngo et Lm. Nutter, STATUS OF GLUTATHIONE AND GLUTATHIONE-METABOLIZING ENZYMES IN MENADIONE-RESISTANT HUMAN CANCER-CELLS, Biochemical pharmacology, 47(2), 1994, pp. 421-424
Citations number
26
Categorie Soggetti
Pharmacology & Pharmacy",Biology
Journal title
ISSN journal
00062952
Volume
47
Issue
2
Year of publication
1994
Pages
421 - 424
Database
ISI
SICI code
0006-2952(1994)47:2<421:SOGAGE>2.0.ZU;2-Q
Abstract
Cloned menadione (MD)-resistant human breast cancer cell lines have be en developed and characterized with respect to glutathione (GSH) conte nt and GSH-metabolizing enzymes. Increases in the activities of gamma- glutamyltranspeptidase and glutathione-S-transferase were demonstrated in the absence of alterations in the GSH content of two cloned MD-res istant cell lines. The MD-resistant cells also displayed alterations i n their growth kinetics, possessing longer doubling times and increase d fractions in the G1/0 phase of the cell cycle as compared to parenta l MD-sensitive cells. The possible mechanisms for the resistance to MD , including an increase in repair of MD-induced DNA damage, are discus sed.