Hm. Heise et al., MULTICOMPONENT ASSAY FOR BLOOD SUBSTRATES IN HUMAN PLASMA BY MIDINFRARED SPECTROSCOPY AND ITS EVALUATION FOR CLINICAL ANALYSIS, Applied spectroscopy, 48(1), 1994, pp. 85-95
An analytical multicomponent method for the blood substrates total pro
tein, glucose, total cholesterol, triglycerides, and urea in human EDT
A plasma by FT-IR spectroscopy is described. The spectra were obtained
with the use of the attenuated total reflection technique. Partial le
ast-squares was applied for multivariate calibration over optimized sp
ectral ranges. The mean-square prediction errors for the population of
126 plasma samples of different patients calculated by cross-validati
on are in the range of clinical acceptance. Within an error variance a
nalysis, the contributions of the reference method and the spectrometr
ic measurement to the average (root mean square) prediction error have
been estimated for each substrate, giving evidence of the limitations
of the spectrometric method. The problem of the biocompatibility of t
he plasma has been investigated, and the protein adsorption onto the A
TR crystal can be reduced to a constant and tolerable level by appropr
iate cleaning and rinsing. The potential for further improvement is di
scussed.