GENDER DETERMINATION BY MULTIPLEX PCR AMPLIFICATION OF ALPHOID REPEATSEQUENCES FROM SINGLE CELLS

Authors
STEUERWALD N LAMBERT H STEINLEITNER AJ HERRERA RJ
Citation
N. Steuerwald et al., GENDER DETERMINATION BY MULTIPLEX PCR AMPLIFICATION OF ALPHOID REPEATSEQUENCES FROM SINGLE CELLS, BioTechniques, 16(1), 1994, pp. 82-84
Citations number
25
Categorie Soggetti
Biochemical Research Methods
Journal title
BioTechniques → ACNP
ISSN journal
07366205
Volume
16
Issue
1
Year of publication
1994
Pages
82 - 84
Database
ISI
SICI code
0736-6205(1994)16:1<82:GDBMPA>2.0.ZU;2-W
Abstract
Alphoid repeat sequences on the X and Y chromosomes were successfully and consistently coamplified to determine the sex of single cells. Buc cal cells sewed as a convenient model for single blastomeres obtained from preimplantation embryos through in vitro fertilization. The poten tial use of this technique for preimplantation generic diagnosis is di scussed.