A RAPID AND EFFICIENT, NONRADIOACTIVE METHOD FOR SCREENING RECOMBINANT-DNA LIBRARIES

Authors
AMARAVADI L KING MW
Citation
L. Amaravadi et Mw. King, A RAPID AND EFFICIENT, NONRADIOACTIVE METHOD FOR SCREENING RECOMBINANT-DNA LIBRARIES, BioTechniques, 16(1), 1994, pp. 98
Citations number
5
Categorie Soggetti
Biochemical Research Methods
Journal title
BioTechniques → ACNP
ISSN journal
07366205
Volume
16
Issue
1
Year of publication
1994
Database
ISI
SICI code
0736-6205(1994)16:1<98:ARAENM>2.0.ZU;2-F
Abstract
In this report we present a rapid and inexpensive PCR-based method to screen recombinant DNA libraries. The efficiency of this method was de monstrated by the isolation of clones of interest from three different libraries using different vector systems. This method is nonradioacti ve and makes it easier to handle a large number of samples since there is no need for DNA extraction. The advantages and applications of the method are discussed.