PROPERTIES AND APPLICATIONS OF A NEW-TYPE OF IODINATED GRADIENT MEDIUM

Authors
BENDIXEN B RICKWOOD D
Citation
B. Bendixen et D. Rickwood, PROPERTIES AND APPLICATIONS OF A NEW-TYPE OF IODINATED GRADIENT MEDIUM, Journal of biochemical and biophysical methods, 28(1), 1994, pp. 43-52
Citations number
6
Categorie Soggetti
Biology,Biophysics,"Biochemical Research Methods
Journal title
Journal of biochemical and biophysical methods → ACNP
ISSN journal
0165022X
Volume
28
Issue
1
Year of publication
1994
Pages
43 - 52
Database
ISI
SICI code
0165-022X(1994)28:1<43:PAAOAN>2.0.ZU;2-#
Abstract
This paper reports on the properties of a new iodinated density-gradie nt medium, Iotrolan, and its potential application for separating dens e cells. Iotrolan is a dimeric, nonionic, iodinated compound which is distinguished by its ability to form dense, non-viscous gradients of l ow osmolarity. Isotonic gradients of up to 1.30 g/ml can be formed, al lowing even dense cells such as sperm to be separated under isotonic c onditions.