Ie. Lee et al., DESMUTAGENICITY OF MELANOIDINS AGAINST VARIOUS KINDS OF MUTAGENS AND ACTIVATED MUTAGENS, Bioscience, biotechnology, and biochemistry, 58(1), 1994, pp. 18-23
The desmutagenic activities of low-molecular weight melanoidins (LM-ME
L, MW 500-1000) and nondialyzable melanoidins (ND-MEL, MW above 1000)
prepared from a glucose-glycine reaction system were examined by Ames'
test on such mutagens/carcinogens as nitro or amino compounds of arom
atics or heterocycles, azo compounds, nitroso compounds, and epoxides.
ND-MEL and LM-MEL exhibited a desmutagenic activity of 25-75% against
mutagenic aromatic or heterocyclic compounds such as aflatoxin B-1, B
[alpha]P, 2-aminofluorene, 4-aminobiphenyl, and 2-aminonaphthalene, as
well as heterocyclic amines, using S. typhimurium TA98 in the presenc
e of S9 mix. In the case of using S. typhimurium TA100 in the absence
of S9 mix, ND-MEL and LM-MEL showed a desmutagenic activity of 20-50%
against 2-nitrofluorene, 4NQO and 2-nitronaphthalene. The strong desmu
tagenic activity of MEL from various kinds of amino acids, peptides, o
r egg albumin hydrolyzates with glucose was also observed against Trp-
P-1. Furthermore, MEL had marked desmutagenic activity against the Trp
-P-1 activated by S9 mix and synthetic N-OH-Trp-P-2.