L. Burgos et Ca. Ledbetter, IMPROVED EFFICIENCY IN APRICOT BREEDING - EFFECTS OF EMBRYO DEVELOPMENT AND NUTRIENT MEDIA ON IN-VITRO GERMINATION AND SEEDLING ESTABLISHMENT, Plant cell, tissue and organ culture, 35(3), 1993, pp. 217-222
Apricot (Prunus armeniaca L.) embryos at three stages of development w
ere cultured on C2d, SBH and WPM media. In vitro culture produced high
percentages of germination and seedlings throughout all three develop
mental stages. Significant media effects were noted for changes in bot
h embryo length and weight during the culture period, as well as numbe
r of plants produced. Embryos between 5 and 9 mm (developmental stage
I) germinated and developed into plants in a significantly higher perc
entage than in the other two more mature stages. Therefore, embryo cul
ture can be successfully used as a tool in an apricot breeding program
to obtain higher percentages of seedlings from planned hybridization
or to overcome a lack of seed germination.