Yc. Qian et al., EFFECT OF WASHING ON THE PLASMA-MEMBRANE AND ON STRESS REACTIONS OF CULTURED ROSE CELLS, Plant cell, tissue and organ culture, 35(3), 1993, pp. 245-252
Cultured cells of Rosa damascena have been used as a model for studies
of responses of plant cells to various stresses, including UV radiati
on, protein-synthesis inhibitors, and elicitors from pathogens. Many o
f the responses involve reactions at the plasma membrane: efflux of K, changes in the acid balance between cytoplasm and external medium, s
ynthesis of H2O2, and inhibition of ferricyanide reduction. In previou
s studies, the cells have typically been washed with a solution of low
ionic strength. We now show that this washing procedure results in ch
anges in the protein composition of the plasma membrane, in the labeli
ng of the proteins in the plasma membrane, and in the specific activit
y of ATPase in purified plasma membrane vesicles. Also, compared to th
e unwashed cells, the washed cells show less net K+ efflux after UV-C
and Phytophthora elicitor treatments; more synthesis of H2O2 after UV-
C and a pattern of accumulation of H2O2 after elicitor treatment that
shows a delayed but higher peak; and more inhibition of ferricyanide r
eduction after UV-C, but not after elicitor treatment. The results sug
gest that washing has differential effects on the mechanisms by which
cultured plant cells perceive or respond to two stresses, UV-C and eli
citor.