M. Mucciarelli et al., CALLUS INDUCTION AND PLANT-REGENERATION IN VETIVERIA ZIZANIOIDES, Plant cell, tissue and organ culture, 35(3), 1993, pp. 267-271
Callus induction was obtained from basal parts of Vetiveria zizanioide
s Stapf. leaves cultured on Murashige and Skoog (MS) medium supplement
ed with 9.0 mu M 2,4-dichlorophenoxyacetic acid (2,4-D), 5.7 mu M indo
leacetic acid (IAA) and 4.6 mu M kinetin. Calli were maintained on MS
medium with the addition of 0.9 mu M 2,4-D and 2.3 mu M kinetin. Shoot
formation was obtained from fast growing 14-day-old callus on the sam
e basal medium supplemented with 0.9 mu M 2,4-D and 9.3 mu M kinetin.
Embryo-like structures were observed. When transferred to basal medium
, shoots readily developed roots. Fully developed regenerated plants w
ere then successfully established in soil.