Bk. Ishida et al., FLAVOR VOLATILES, SUGARS AND COLOR DEVELOPMENT IN RIPENING IN-VITRO-CULTURED TOMATO FRUIT AND CALYX, Physiologia Plantarum, 89(4), 1993, pp. 861-867
Previous studies showed that the developmental program of calyces of a
tomato cultivar (Lycopersicon esculentum, cv. VFNT Cherry) changed in
many aspects to that of fruit when cultured in vitro. The calyces tur
ned red, produced ethylene, had increased tissue content of 1-aminocyc
lopropane-1-carboxylic acid, had increased levels of the mRNA of polyg
alacturonase and developed ultrastructural changes in their cell walls
that were indistinguishable from those of ripe tomato fruit tissue. W
e report in the present study the synthesis of volatile flavor compoun
ds, changes in sugar concentrations and color development in cultured
calyces that are characteristic of ripening tomato fruit. These ripeni
ng parameters of in vitro-cultured tomato fruit were also compared to
those of fruit grown in the greenhouse.