NEURONS DERIVED FROM EMBRYONAL CARCINOMA (P19) CELLS EXPRESS MULTIPLEGABA(A) RECEPTOR SUBUNITS AND FULLY FUNCTIONAL GABA(A) RECEPTORS

Authors
REYNOLDS JN RYAN PJ PRASAD A PATERNO GD
Citation
Jn. Reynolds et al., NEURONS DERIVED FROM EMBRYONAL CARCINOMA (P19) CELLS EXPRESS MULTIPLEGABA(A) RECEPTOR SUBUNITS AND FULLY FUNCTIONAL GABA(A) RECEPTORS, Neuroscience letters, 165(1-2), 1994, pp. 129-132
Citations number
23
Categorie Soggetti
Neurosciences
Journal title
Neuroscience letters → ACNP
ISSN journal
03043940
Volume
165
Issue
1-2
Year of publication
1994
Pages
129 - 132
Database
ISI
SICI code
0304-3940(1994)165:1-2<129:NDFEC(>2.0.ZU;2-0
Abstract
The embryonal carcinoma (EC) cell line P19 can be induced to different iate into neurons and glia by treatment with retinoic acid. Neuronal c ells derived from P19 stem cells were found to express messenger RNAs for alpha, beta, and gamma(2) subunits of the GABA(A) receptor-chlorid e channel complex. Whole-cell voltage-clamp recording in differentiate d P19 cells revealed that these cells possess GABA receptor-activated chloride currents which are blocked by bicuculline and potentiated by flurazepam. P19 EC cells thus represent a stable neuronal cell line wh ich expresses functional GABA(A) receptors with all of the characteris tics of native GABA(A) receptors.