Quantitative bacteriology is presented on 102 consecutive split-skin g
rafts. A sample of graft was cultured whenever skin was taken and when
ever stored skin was used. Stored skin unused at 21 days was also cult
ured. The percentage take of grafts was inversely proportional to numb
er of organisms/g of skin (r = - 0.24, p < 0.05). Commonly used storag
e conditions facilitated bacterial multiplication. There was no signif
icant difference in organisms/g contaminating grafts for different sur
geons, skin preparations, types of grafts, cutting tools or mode of an
aesthesia. Male patients had a significantly greater count for initial
grafts (p = 0.02), but after 3 weeks storage there was no sex differe
nce.