STIMULATION OF HUMAN IGE PRODUCTION BY A SUBSET OF ANTI-CD21 MONOCLONAL-ANTIBODIES - REQUIREMENT OF A COSIGNAL TO MODULATE EPSILON-TRANSCRIPTS

CD21, the receptor for Epstein-Barr virus (EBV) and the complement rec eptor-2 (CR2), was recently found to interact specifically with CD23, a low-affinity receptor for IgE, and to regulate IgE production. There fore, the effect of different anti-CD21 monoclonal antibodies (mAb) on IgE synthesis by blood mononuclear cells was investigated. One anti-C D21 mAb, BU-33, was able to increase significantly (more than threefol d) interleukin-4 (IL-4)-induced IgE synthesis, whereas HB-5, OKB-7 and B2 anti-CD21 mAb had no effect. BU-33 had no effect on IgG and IgA pr oduction and produced only a moderate increase in IgM production. Reco mbinant, 29,000 MW, soluble CD23 (sCD23) expressed in COS cells exhibi ted the same IgE-enhancing activity. BU-33 was the best inhibitor of C D23-liposome binding to the CD21-positive cell line RPMI-8226 when com pared to the other anti-CD21 mAb tested. BU-33 identified a different epitope on CD21. The effect of BU-33 on IgE production by purified ton sillar B cells and highly purified germinal centre B cells, was depend ent on the presence of T cells or anti-CD40 mAb stimulation. Molecular analysis revealed that BU-33 alone failed to induce germline epsilon mRNA but increased the IL-4-induced germline epsilon transcription lev els. Moreover, BU-33 had a synergistic effect on anti-CD40 mAb or T-ce ll-induced productive E transcript expression. These results therefore indicate that the CD23-CD21 interaction needs a co-signal for B-cell differentiation towards IgE production.