SEPARATION OF THE MAIN NEUTRAL LIPIDS INTO CLASSES AND SPECIES BY PR-HPLC AND UV DETECTION

A reversed phase high performance liquid chromatographic method is des cribed that allows separation and estimation of the main neutral lipid classes as well as several species of each class. By the represented method waxes, hydrocarbons, fatty acids and their methyl esters, stero ls and their esters, free glycerylethers, fatty alcohols, vitamin E an d mono-, di- and triglycerides are separated into classes and into cla ss species within 55min. A stepped gradient elution with methanol/wate r, acetonitrile/methanol, acetonitrile/tetrahydrofuran and isopropanol /acetonitrile was performed onto a reverse phase C18 HPLC column and t he effluent was monitored by W detection at 206 nm. Application of the method in a plant extract and in a vegetable oil sample is represente d.