CARBOXYMETHYLDEXTRAN LACTONE - A PREACTIVATED POLYMER FOR AMINE CONJUGATIONS

Authors
HEINDEL ND KAUFFMAN MA AKYEA EK ENGEL SA FREY MF LACEY CJ EGOLF RA
Citation
Nd. Heindel et al., CARBOXYMETHYLDEXTRAN LACTONE - A PREACTIVATED POLYMER FOR AMINE CONJUGATIONS, Bioconjugate chemistry, 5(1), 1994, pp. 98-100
Citations number
15
Categorie Soggetti
Biology,Chemistry
Journal title
Bioconjugate chemistry → ACNP
ISSN journal
10431802
Volume
5
Issue
1
Year of publication
1994
Pages
98 - 100
Database
ISI
SICI code
1043-1802(1994)5:1<98:CL-APP>2.0.ZU;2-O
Abstract
The linking of amino haptens to carboxymethyldextran (CMD) requires ca rboxyl activation, for example, via carbodiimides. We have discovered that substantial N-acylurea, derived from these carbodiimides, can be trapped on the CMD backbone. As an alternative, CMD can be convenientl y lactonized by heating in inert solvents, and this carboxymethyldextr an lactone (CDL) can be employed directly for amine conjugation.