A. Meier et al., GENETIC ALTERATIONS IN STREPTOMYCIN-RESISTANT MYCOBACTERIUM-TUBERCULOSIS - MAPPING OF MUTATIONS CONFERRING RESISTANCE, Antimicrobial agents and chemotherapy, 38(2), 1994, pp. 228-233
We report on the identification of mutations associated with streptomy
cin resistance in Mycobacterium tuberculosis. Two isolates (3656 and 3
976) showed a wild-type ribosomal protein, S12, but exhibited a single
point mutation at 168 rRNA position 491 (C-->T) or 512 (C-->T), respe
ctively. Sequence analysis of a third isolate (2438) revealed a single
base change at 168 rRNA position 904 (A-->G). This position is equiva
lent to invariant position 913 of the Escherichia coli 168 rRNA gene,
an A-->G transition of which has been shown previously to impair strep
tomycin binding and streptomycin-induced misreading in vitro. Surprisi
ngly, strain 2438 harbors an additional mutation in the ribosomal prot
ein S12 (Lys 88-->Gln).