EXPRESSION OF REVERSE-TRANSCRIPTASE FROM FELINE IMMUNODEFICIENCY VIRUS IN ESCHERICHIA-COLI

Authors
NORTH TW HANSEN GL ZHU YQ GRIFFIN JA SHIH CK
Citation
Tw. North et al., EXPRESSION OF REVERSE-TRANSCRIPTASE FROM FELINE IMMUNODEFICIENCY VIRUS IN ESCHERICHIA-COLI, Antimicrobial agents and chemotherapy, 38(2), 1994, pp. 388-391
Citations number
17
Categorie Soggetti
Pharmacology & Pharmacy",Microbiology
Journal title
Antimicrobial agents and chemotherapy → ACNP
ISSN journal
00664804
Volume
38
Issue
2
Year of publication
1994
Pages
388 - 391
Database
ISI
SICI code
0066-4804(1994)38:2<388:EORFFI>2.0.ZU;2-K
Abstract
Reverse transcriptase from feline immunodeficiency virus (FIV) has bee n cloned and expressed in Escherichia coli. We have purified this reco mbinant enzyme and shown that it is a 66-kDa protein that is indisting uishable from virion-derived FIV reverse transcriptase in sensitivity to the 5'-triphosphates of 3'-azido-3'-deoxythymidine and the four 2', 3'-dideoxynucleosides. The availability of large quantities of the FIV reverse transcriptase will allow more detailed physical and pharmacol ogical studies.