ANOPHELES CULICIFACIES - EFFECTS OF AGE ON THE MALE REPRODUCTIVE-SYSTEM AND MATING ABILITY OF VIRGIN ADULT MOSQUITOS

Under controlled laboratory conditions of 28-30 degrees C and 16:8 L:D photoperiod, an attempt was made to develop an age-grading technique for Anopheles culicifacies males. Mating activity was maximal when fem ales were 5-12 days old and males were 5-7 days old. The numbers of to tal and mature spermatocysts declined significantly with age, and the proportion of the testes occupied by the sperm reservoir increased as virgin males grew older. Mating resulted in the loss of spermatozoa an d accessory gland substance from the reproductive system. Loss of mati ng ability of older virgin males seemed to be age-related, because the reproductive system contained ample supplies of accessory gland subst ance and spermatozoa. Morphological changes of the reproductive system , due to mating and age, were used to infer the age and reproductive h istory of unknown males in a laboratory evaluation.