T. Kondo et al., ENHANCED PHOSPHOLIPASE-D ACTIVITY IN VASCULAR SMOOTH-MUSCLE CELLS DERIVED FROM SPONTANEOUSLY HYPERTENSIVE RATS, Clinical and experimental hypertension, 16(1), 1994, pp. 17-28
Citations number
31
Categorie Soggetti
Pharmacology & Pharmacy","Cardiac & Cardiovascular System
When cultured in the presence of fetal calf serum, aortic vascular smo
oth muscle cells (VSMC) derived from spontaneously hypertensive rats (
SHR) grow faster than those from normotensive control Wistar-Kyoto (WK
Y) rats. In order to investigate the mechanism underlying this growth
abnormality, we measured phospholipase D (PLD) activity in VSMC taken
from both SHR and WKY rats. Upon stimulation with serum, platelet-deri
ved growth factor (PDGF) and porbol 12-myristate 13-acetate (TPA), pho
sphatidylethanol (PEt) was produced in the presence of ethanol. The re
sponses of the VSMC from SHR (SHR-cells) to all stimuli were significa
ntly greater than those of the VSMC from WKY rats (WKY-cells), which s
uggests an enhanced PLD activity in the SHR-cells. Since PLD is regard
ed as an enzyme involved in signal transduction leading to cell prolif
eration, this PLD hyper-reactivity in the SHR-cells may account at lea
st partially for the growth abnormality in the SHR-cells.