INHIBITION OF HORMONE-STIMULATED INOSITOL PHOSPHATE PRODUCTION AND DISRUPTION OF CYTOSKELETAL STRUCTURE - EFFECTS OF OKADAIC ACID, MICROCYSTIN, CHLORPROMAZINE, W7 AND NYSTATIN

Authors
MACIASSILVA M GARCIASAINZ JA
Citation
M. Maciassilva et Ja. Garciasainz, INHIBITION OF HORMONE-STIMULATED INOSITOL PHOSPHATE PRODUCTION AND DISRUPTION OF CYTOSKELETAL STRUCTURE - EFFECTS OF OKADAIC ACID, MICROCYSTIN, CHLORPROMAZINE, W7 AND NYSTATIN, Toxicon, 32(1), 1994, pp. 105-112
Citations number
33
Categorie Soggetti
Toxicology,"Pharmacology & Pharmacy
Journal title
Toxicon → ACNP
ISSN journal
00410101
Volume
32
Issue
1
Year of publication
1994
Pages
105 - 112
Database
ISI
SICI code
0041-0101(1994)32:1<105:IOHIPP>2.0.ZU;2-O
Abstract
Inhibition of protein phosphatases 2A and 1 by okadaic acid and microc ystin leads to cytoskeletal disruption and formation of plasma membran e blebs (blebbing) in hepatocytes. This phenomenon is associated to a marked inhibition of receptor-mediated and G-protein-mediated phosphoi nositide turnover in rat hepatocytes. Other cytoskeletal-disrupting dr ugs such as chlorpromazine, W7 and nystatin mimic the effect of these protein phosphatase inhibitors on phosphoinositide metabolism and bleb bing. Our data suggest that the coupling between G-protein and phospho lipase C might be altered by cytoskeletal disruption.