Ixodes ricinus ticks were collected by flagging vegetation of a mixed
oak forest in South Moravia (Czech Republic) at regular two-month inte
rvals from March 1991 to March 1992 and examined for borreliae by dark
field microscopy. Mean annual proportions of infected ticks were 17.2%
(15.4% to 21.2% monthly) in females (F), 18.6% (11.8% to 25.9%) in ma
les (M), and 16.3% (12.4% to 20.9%) in nymphs (N); the differences amo
ng monthly values were insignificant. However, monthly proportions of
intensively infected ticks containing more than 100 borreliae fluctuat
ed widely, from 0.0% to 7.7% (annual mean 3.3%) in F, from 0.0% to 5.6
% (mean 2.4%) in M, and from 0.0% to 5.7% (mean 1.9%) in N; the differ
ences among months were significant, and the peak prevalence values we
re found in July (F, M) or November (N). Mean numbers of borreliae per
infected tick reached their peak in September for both adult and nymp
hal ticks while they were generally low in spring. The highest risk of
infection with tick-borne borreliae for vertebrates (including man) o
ccurred in the study area during the month of July; in that month, one
either female or nymphal I. ricinus containing more than 100 borrelia
e was encountered, on the average, every 92 minutes of flagging.