EVIDENCE THAT GABA, SEROTONIN, AND NOREPINEPHRINE ARE INVOLVED IN THEMODULATION OF IN-VITRO RHYTHMICAL ACTIVITY IN RAT HIPPOCAMPAL SLICES

Cholinergic agonists induce a rhythmical slow activity (RSA) in the in vitro rat hippocampus. RSA consists of bursts of activity separated b y quiescent periods (interburst intervals). The activity involves acti vation of muscarinic receptors; however, the role of other neurotransm itter substances is still controversial. The present study demonstrate s that 500 mu M GABA, 15 mu M serotonin (5HT), or 20 mu M norepinephri ne (NE) can alter the pattern of carbachol-induced RSA. Application of GABA, 5HT, or NE increases interburst interval; 5HT and NE also incre ase burst length. Total power of RSA is decreased by GABA and 5HT but increased by NE. None of the three receptor agonists alters RSA freque ncy. The pattern of RSA is also dependent upon carbachol concentration : low concentrations (0.5 and 1 mu M) produce only population spikes, whereas concentrations of 3 to 100 mu M produce burst activity (50 mu M is optimal for the generation of RSA). Burst length and peak frequen cy of RSA are enhanced with increasing concentrations of carbachol, wh ereas interburst interval is decreased. The results illustrate that th e pattern of RSA is not only dependent upon carbachol concentration bu t can be modulated by GABA, 5HT, and NE. This suggests that more than one neurotransmitter system contributes to the production and modulati on of RSA.