COMPARATIVE INVESTIGATIONS INTO THE DETEC TION OF BHV-5 ANTIBODIES ENZYME-LINKED-IMMUNOSORBENT-ASSAY, SERUM NEUTRALIZATION TEST, CELL ASSAYAND IMMUNOFLUORESCENCE ASSAY

Authors
LINDNER A AMBROSIUS H LIEBERMANN H
Citation
A. Lindner et al., COMPARATIVE INVESTIGATIONS INTO THE DETEC TION OF BHV-5 ANTIBODIES ENZYME-LINKED-IMMUNOSORBENT-ASSAY, SERUM NEUTRALIZATION TEST, CELL ASSAYAND IMMUNOFLUORESCENCE ASSAY, DTW. Deutsche tierarztliche Wochenschrift, 100(11), 1993, pp. 440-442
Citations number
20
Categorie Soggetti
Veterinary Sciences
Journal title
DTW. Deutsche tierarztliche Wochenschrift → ACNP
ISSN journal
03416593
Volume
100
Issue
11
Year of publication
1993
Pages
440 - 442
Database
ISI
SICI code
0341-6593(1993)100:11<440:CIITDT>2.0.ZU;2-O
Abstract
A comparison of the analyses of 490 serum samples from various flocks of sheep for the detection of BHV-5 antibodies using ELISA, serum neut ralisation test, cell assay and immunofluorescence assay showed nearly identical results regarding the distinction between positive and nega tive serum samples. The percentage of serum samples with antibodies ag ainst BHV-5 antigen differed from test to test: ELISA 268 (54,7 %), ce ll assay 323 (65,8 %), immunofluorescence assay 222 (46,6 %) and serum neutralisation test 221 (45,1 %). With ELISA, cell assay and immunofl uorescence assay the experiment can be carried out more simply and rap idly than with serum neutralisation test.