KINETIC-STUDY OF THE PERFORMANCE OF 3RD-GENERATION BIOSENSORS

A kinetic study of the performance of third-generation biosensors for glucose based on glucose oxidase immobilized on a microporous matrix o f the conducting polymer poly(pyrrole) is presented. The mechanism of the enzymatically catalyzed oxidation of glucose will be different in this type of biosensor as the natural electron acceptor oxygen is repl aced by the conducting polymer. Different kinetic parameters are found for the immobilized glucose oxidase than for the enzyme in solution. Mediation by the conducting polymer is found to be very effective and no significant electron transfer to oxygen is observed. In addition to substrate transport limitation in the microporous matrix, the enzymat ic reaction in the biosensors is limited by the applied potential.