Cw. Chan et I. Suzuki, QUANTITATIVE EXTRACTION AND DETERMINATION OF ELEMENTAL SULFUR AND STOICHIOMETRIC OXIDATION OF SULFIDE TO ELEMENTAL SULFUR BY THIOBACILLUS THIOOXIDANS, Canadian journal of microbiology, 39(12), 1993, pp. 1166-1168
A sensitive and quantitative analytical method for determining element
al sulfur in a biological system was developed. Elemental sulfur was d
etermined after extraction with petroleum ether by cyanolysis and ferr
ic thiocyanate color formation in acetone. The method was successfully
applied to show that sulfide was oxidized by Thiobacillus thiooxidans
to elemental sulfur nearly stoichiometrically when further oxidation
of elemental sulfur was inhibited by N-ethylmaleimide.