The United States National Plant Germplasm System contains 3159 access
ions from 36 species of annual Medicago. Although there is increasing
interest in the annual medics for use in sustainable agriculture, the
U.S. collection is under utilized because of lack of agronomic informa
tion. Development of a core collection could facilitate easier access
to the germplasm collection and enhance its use. The core collection s
hould consist of a sample of accessions that represents the range of v
ariability within the germplasm collection with minimum redundancies.
To select a core collection of annual Medicago species, a subset of 12
40 accessions was evaluated during the summer of 1990 for 16 agronomic
and morphological traits. Accessions within species were grouped by c
luster analysis utilizing an unweighted pair group method with arithme
tic averages. Intraspecific phenotypic diversity determined the number
of accessions for that species selected for the core collection. One
accession per cluster was selected for each species for the core colle
ction. Accessions were chosen within a species to represent the greate
st diversity in geographical regions. The selected core collection of
211 accessions was reevaluated during the summer of 1991. The core col
lection was found to represent the variability of the germplasm collec
tion and to remain stable between the two evaluation years. This study
can be used as a model method for selecting a core collection for mul
tispecies germplasm collections.