PRODUCTION OF DOUBLED-HAPLOID PLANTS FROM TRITORDEUM ANTHER CULTURE

The effects of different media and cold pretreatment of spikes on the androgenic response and regeneration capacity from anther culture of t ritordeum was studied. L5 medium gave the highest frequency of anther response. The frequency of cultures regenerating green or albino plant lets was not affected by the composition of the medium tested. Cold pr etreatment of the spikes significantly increased the frequency of anth er response and also the percentage of cultures giving albino plantlet s. A mean of four green plants was obtained per 100 subcultured calli/ embryos. The percentage of spontaneous chromosome doubling was only 1% . The addition of colchicine at 0.02% to the induction medium signific antly increased the frequency of doubled haploids regenerated without any effect on regeneration capacity. This technique proved more effici ent than a conventional chromosome-doubling method.