PURIFICATION AND CELLULOSOMAL LOCALIZATION OF CLOSTRIDIUM-THERMOCELLUM MIXED LINKAGE BETA-GLUCANASE LICB (1,3-1,4-BETA-D-GLUCANASE)

Authors
ZVERLOV VV FUCHS KP SCHWARZ WH VELIKODVORSKAYA GA
Citation
Vv. Zverlov et al., PURIFICATION AND CELLULOSOMAL LOCALIZATION OF CLOSTRIDIUM-THERMOCELLUM MIXED LINKAGE BETA-GLUCANASE LICB (1,3-1,4-BETA-D-GLUCANASE), Biotechnology letters, 16(1), 1994, pp. 29-34
Citations number
18
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
16
Issue
1
Year of publication
1994
Pages
29 - 34
Database
ISI
SICI code
0141-5492(1994)16:1<29:PACLOC>2.0.ZU;2-6
Abstract
Clostridium thermocellum recombinant beta-(1,3-1,4)-glucanase LicB was expressed in Escherichia coli K-12 and purified to electrophoretic ho mogeneity. Specific activity of the final preparation was 12,000 U/mg. Antibodies against recombinant LicB protein reacted with a specific b and of purified C. thermocellum F7 cellulosomes, suggesting localizati on in the extracellular multienzyme complex. This indicates that the c ellulosome takes part in the hydrolysis of other polysaccharides besid es cellulose.