Ar. Sousa et al., INCREASED EXPRESSION OF THE MONOCYTE CHEMOATTRACTANT PROTEIN-1 IN BRONCHIAL TISSUE FROM ASTHMATIC SUBJECTS, American journal of respiratory cell and molecular biology, 10(2), 1994, pp. 142-147
The expression of the monocyte chemoattractant protein (MCP-1), a memb
er of the chemokine family of low molecular weight cytokines, was asse
ssed by immunohistochemistry in bronchial biopsies from 12 asthmatic a
nd 12 normal subjects. Both a monoclonal antibody (F9) and a polyclona
l antibody were employed to detect MCP-1, while the mouse myeloma prot
ein (MOPC21) was used as a negative control. Strong positive reactions
for MCP-1 were seen in the bronchial epithelium. Subepithelial macrop
hages, blood vessels, and bronchial smooth muscle were also stained. H
ue-saturation-intensity color image analysis was used to quantify reac
tions of the monoclonal antibody in the epithelial and subepithelial l
ayers. With the monoclonal antibody, asthmatic biopsies showed 51.8 +/
- 3.7% (mean +/- SEM) of the epithelium staining positively, whereas n
ormal subjects reacted much less, with 6.4 +/- 1.9% of the epithelium
staining (P < 0.0001); there was no overlap between the two groups. Li
kewise, staining was increased in the subepithelium of asthmatic airwa
y biopsies, with 11.5 +/- 3.1% and 2.0 +/- 1.0% staining positively in
asthmatic and normal subepithelium, respectively, (P < 0.002). There
was a significant correlation between staining of the epithelium and s
ubepithelium (r = 0.77, P < 0.001). The polyclonal anti-MCP-1 antibody
also gave strong reactions in the epithelium and subepithelium, with
34.0 +/- 7.8% of the asthmatic and 1.6 +/- 1.0% of the normal bronchia
l epithelium staining positively (P < 0.0001). These increased levels
of MCP-1 in the asthmatic airways suggest that they may play a role in
macrophage recruitment and activation and thereby contribute to the i
nflammatory pathology of bronchial asthma.