PREPARATION OF MAGNETIC SUPPORT FOR GLUTA MINASE IMMOBILIZATION - (A STUDY ON THE CONVERSION OF GLUTAMINE TO GLUTAMIC-ACID BY AN IMMOBILIZED GLUTAMINASE ON A SUPPORT HAVING MAGNETIC SENSITIVITY .1.)

Authors
KOSEKO S HISAMATSU M YAMADA T
Citation
S. Koseko et al., PREPARATION OF MAGNETIC SUPPORT FOR GLUTA MINASE IMMOBILIZATION - (A STUDY ON THE CONVERSION OF GLUTAMINE TO GLUTAMIC-ACID BY AN IMMOBILIZED GLUTAMINASE ON A SUPPORT HAVING MAGNETIC SENSITIVITY .1.), J JPN SOC F, 41(1), 1994, pp. 31-36
Citations number
NO
Categorie Soggetti
Food Science & Tenology
Journal title
JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI
ISSN journal
1341027X → ACNP
Volume
41
Issue
1
Year of publication
1994
Pages
31 - 36
Database
ISI
SICI code
1341-027X(1994)41:1<31:POMSFG>2.0.ZU;2-P
Abstract
In order to recover glutaminase added to koji hydrolysate before alcoh ol fermentation through bioreactor, we investigated the enzyme immobil ized on a magnetic support prepared from partially deacetylated chitin (PDAC), chitosan and Fe3O4. The magnetic support (MC4P) was superior in endurance to high salt concentration in the solution over a long pe riod. The MC4P was easily recovered by magnetic force. Glutaminase (15 U) immobilized on MC4P (Ig) under suitable preparation condition expre ssed 100% enzyme activity compared to free enzyme. Glutaminase activit y immobilized on MC4P was stable in the 15% NaCl solution for 36 days.