EXPRESSION OF A GENE (IDS2) INDUCED BY FE DEFICIENCY TREATMENT IN THEROOTS OF HORDEUM-VULGARE L

A lambda ZAPII-cDNA library constructed from poly(A)(+)-RNA isolated f rom Fe-deficient barley roots was used for differential screening of b arley roots grown in the presence and absence of Fe. Among seven clone s that hybridised specifically to the probe for Fe deficiency, one clo ne (Ids2) was sequenced. Using a part of the cDNA sequence as a probe, a genomic-DNA library was probed and a corresponding DNA clone was is olated and sequenced. The predicted amino acid sequence resembled 2-ox oglutarate-dependent dioxygenase. Ids2 had a metal regulatory element as well as Cu regulatory elements of CUP1 gene of yeast MT.