IN-VITRO REGENERATION OF ARACHIS-VILLOSULICARPA HOEHNE FROM COTYLEDONSEGMENTS, LEAVES AND CELL-SUSPENSION

In vitro regeneration from immature leaves and cotyledon segments of A . villosulicarpa Hoehne was obtained on Murashige and Skoog medium (19 62) plus 1.0 mg/l 1-naphthalene acetic acid (NAA) and 1.0 mg/l 6-benzy laminopurine (BAP). Cotyledon segments showed higher morphogenetic pot ential than immature leaves. Morphogenic calli could also be obtained from leaves of regenerated plants propagated in vitro. Cell suspension cultures were established from friable calli originated from leaf and cotyledon explants cultured on MS medium supplemented with 0.2 mg/l p icloram and 1.0 mg/l BAP. Calli from liquid culture developed shoots o n regeneration medium. Root differentiation of plantlets was achieved on liquid MS medium.