RADIOPROTECTIVE EFFICIENCY OF A COMBINATION OF HYDROXYTRYPTOPHAN AND AET ON MOUSE BONE-MARROW MICRONUCLEI

Radioprotective effectiveness of a combination of HT and AET was evalu ated by radiation induced micronucleus screening in mouse bone marrow. Swiss albino mice were injected with 2-Aminoethylisothiouronium bromi de hydrobromide (AET)+5-hydroxy-L-tryptophan (HT) 30 minutes prior to a single whole body exposure of 8 and 12 Gy Co-60 gamma rays. Animals were sacrificed at different intervals viz., 24 hours, 40 hours, 72 ho urs and 7 days after irradiation. Slides of femur marrow were screened thoroughly for cells with micronuclei. Marked decreases in the freque ncy of micronuclei in irradiated mice receiving radioprotector suggest ed that the combination has rendered protection to bone marrow cells a gainst 8 Gy and 12 Gy whole body gamma irradiation. Micronucleated cel ls count is not altered by administration of the combination alone.