PURIFICATION AND PROPERTIES OF 2 CHITINASES FROM VIBRIO-ALGINOLYTICUSH-8

Chitinases C1 and C3 from Vibrio alginolyticus H-8 were purified by co lumn chromatography on DEAE-Toyopearl 650M and Superdex 200HR. The mol ecular weights were estimated by SDS-gel electrophoresis (SDS-PAGE) to be 81,000 Da and 68,000 Da for C1 and C3, respectively. The pIs for C 1 sad C3 were 3.9 and 3.6, respectively. The activities of both enzyme s were inhibited by Ag+ and Ng(2+).