INTERACTIONS OF INTERFERON AND VINBLASTINE ON EXPERIMENTAL TUMOR-MODEL MELANOMA-B-16 IN-VITRO

In this study, we tried to define in vitro interactions of two antitum or agents that have different sites and different mechanisms of action . Vinblastine (VLB) in combination with human recombinant interferon-a lpha A/D (rHuIFN-alpha A/D) and in combination with murine recombinant interferon-gamma (rMuIFN-gamma) was studied. The effect of the combin ation was determined with cell growth kinetics assay on B-16 melanoma and the interaction defined by means of Spector's formula. Both the co mbination of rHuIFN-alpha A/D with VLB and the combination of rMuIFN-g amma with VLB synergistically inhibited cell growth in vitro. There wa s a positive biochemical modulation between the two drugs, but it is s till unknown whether it occurred at the level of uptake into the cell, metabolism within the cell or egress from the cell.