SYNTHESIS OF ALPHA-ANOMER AND BETA-ANOMER OF UDP-N-ACETYLMURAMIC ACID

Authors
BLANOT D AUGER G LIGER D VANHEIJENOORT J
Citation
D. Blanot et al., SYNTHESIS OF ALPHA-ANOMER AND BETA-ANOMER OF UDP-N-ACETYLMURAMIC ACID, Carbohydrate research, 252, 1994, pp. 107-115
Citations number
29
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Carbohydrate research → ACNP
ISSN journal
00086215
Volume
252
Year of publication
1994
Pages
107 - 115
Database
ISI
SICI code
0008-6215(1994)252:<107:SOAABO>2.0.ZU;2-A
Abstract
A convenient synthesis of UDP-N-acetylmuramic acid, a key compound for the study of the cytoplasmic synthetases of bacterial peptidoglycan, is described. Separation of its two anomers was carried out by HPLC. T he alpha anomer is identical with natural UDP-N-acetylmuramic acid. Bo th anomers are substrates for the L-alanine-adding enzyme from Escheri chia coli with K-m values of 0.13mM (alpha) and 2 mM (beta).