H. Masoud et Jc. Richards, STRUCTURAL ELUCIDATION OF THE SPECIFIC CAPSULAR POLYSACCHARIDE OF RHODOCOCCUS-EQUI SEROTYPE-7, Carbohydrate research, 252, 1994, pp. 223-233
Structural analysis of the specific capsular polysaccharide produced b
y Rhodococcus equi serotype 7 indicated it to be a high-molecular-weig
ht polymer consisting of equal molar amounts of D-galactose, D-mannose
, L-rhamnose, and pyruvic acid. By employing a combination of chemical
and NMR techniques, it was established that the polysaccharide is com
posed of the linear repeating trisaccharide units, [GRAPHICS] in which
the cyclic pyruvic acid acetal groups bridging the O-4 and O-6 positi
ons of the alpha-D-Man p residues have the S-configuration. The H-1 an
d C-13 NMR spectra of the native and pyruvic acetal-free polysaccharid
es were fully assigned.