IN-VITRO SUSCEPTIBILITY OF FUNGAL ISOLATES OF CLINICALLY IMPORTANT SPECIMENS TO ITRACONAZOLE, FLUCONAZOLE AND AMPHOTERICIN-B

The in vitro activity of itraconazole, fluconazole and amphotericin B was tested against 207 yeast strains and 3 Aspergillus fumigatus strai ns isolated from blood. The other 42A. fumigatus strains were selected from respiratory tract infections. A microdilution method was employe d to determine the inhibitory concentrations to restrain 70% of isolat e growth (IC30). The inhibition concentrations of amphotericin B are a ll around the value 0.25 mg/l with a deviation of two concentration de grees, indicating sensitivity of all strains. The test results of itra conazole showed the majority of strains in the range of sensitivity (2 00 isolates) and low sensitivity (44 isolates). Five of 19 Candida gla brata strains demonstrated resistance to itraconazole. The results of fluconazole showed a good sensibility of C. albicans, but 4.5% of stra ins were resistant. Low sensitivity (1 strain) and resistance (24 stra ins) were evaluated for C. glabrata and C. krusei isolates. No strain of A. fumigatus; was inhibited by fluconazole. The data show the neces sity of in vitro tests.