RAPID DETECTION OF PCR PRODUCTS OF HEPATITIS-C VIRUS (HCV) WITH A NONRADIOACTIVE OLIGOPROBE

Authors
CANEPARI P DISTEFANO CF LLEO MM
Citation
P. Canepari et al., RAPID DETECTION OF PCR PRODUCTS OF HEPATITIS-C VIRUS (HCV) WITH A NONRADIOACTIVE OLIGOPROBE, The New microbiologica, 17(1), 1994, pp. 9-14
Citations number
NO
Categorie Soggetti
Microbiology
Journal title
The New microbiologica
ISSN journal
11217138 → ACNP
Volume
17
Issue
1
Year of publication
1994
Pages
9 - 14
Database
ISI
SICI code
1121-7138(1994)17:1<9:RDOPPO>2.0.ZU;2-J
Abstract
Dot-blot for rapid detection of PCR amplification products of hepatiti s C virus (HCV) using a digoxigenin (DIG)-labelled oligoprobe was deve loped and its sensitivity compared with Southern blot hybridization. T he specificity and sensitivity of the DIG-labelled probe were identica l to those of the 32P-labelled when the DIG-labelled nucleic acids wer e detected by enzyme-catalyzed chemiluminescent reaction. The lack of radioactivity makes this procedure suitable for routine use in diagnos tic laboratories.