SEROTYPIC DIFFERENTIATION OF ROTAVIRUSES IN-FIELD SAMPLES FROM DIARRHEIC PIGS BY USING NUCLEIC-ACID PROBES SPECIFIC FOR PORCINE VP4 AND HUMAN AND PORCINE VP7 GENES
Bi. Rosen et al., SEROTYPIC DIFFERENTIATION OF ROTAVIRUSES IN-FIELD SAMPLES FROM DIARRHEIC PIGS BY USING NUCLEIC-ACID PROBES SPECIFIC FOR PORCINE VP4 AND HUMAN AND PORCINE VP7 GENES, Journal of clinical microbiology, 32(2), 1994, pp. 311-317
Of 216 fecal and intestinal samples collected from nursing or weaned d
iarrheic pigs in the United States and Canada, 57 were identified as g
roup A rotavirus positive by RNA electrophoresis and silver staining.
Fifty-seven and 52 rotavirus-positive samples were analyzed by hybridi
zation with Gottfried and OSU PCR-derived gene 9 and 4 probes, respect
ively. Only 17 samples were identified with either homologous VP4 (P)
or VP7 (G)-coding genes or both. One rotavirus identified as G4 and P7
was similar to the previously characterized interserotype rotavirus,
SB-1A. Additional hybridization analyses were performed with PCR-deriv
ed probes prepared from gene 9 cDNA of the human rotaviruses Wa (G1),
DS-1 (G2), and P (G3) and of the porcine rotavirus YM (G11). Eleven of
52 samples collected and analyzed from swine in Ohio, California, and
Nebraska were identified as G11. No samples with G1-, G2-, or G3-type
specificities were detected among the 25 of 57 rotavirus-positive sam
ples analyzed with human rotavirus-derived probes. Further investigati
ons with a PCR-derived gene 4 probe prepared from porcine rotavirus YM
revealed hybridization specificities similar to those of the OSU gene
4 probe.