F. Hofer et al., MEMBERS OF THE LOW-DENSITY-LIPOPROTEIN RECEPTOR FAMILY MEDIATE CELL ENTRY OF A MINOR-GROUP COMMON COLD VIRUS, Proceedings of the National Academy of Sciences of the United Statesof America, 91(5), 1994, pp. 1839-1842
A protein binding to a minor-group human rhinovirus (HRV2) was purifie
d from HeLa cell culture supernatant. The amino acid sequences of tryp
tic peptides showed identity with the human low density lipoprotein (L
DL) receptor (LDLR). LDL and HRV2 mutually competed for binding sites
on human fibroblasts. Cells down-regulated for LDLR expression yielded
much less HRV2 upon infection than cells with up-regulated LDLR. Viru
s also bound to the large subunit of the alpha(2)-macroglobulin recept
or/LDLR-related protein (alpha(2)MR/LRP). LDLR-deficient fibroblasts y
ielded considerably less virus in the presence of receptor-associated
protein (RAP), providing evidence that alpha(2)MR/LRP also acts as a m
inor group HRV receptor.