G. Delsal et al., STRUCTURE, FUNCTION, AND CHROMOSOME MAPPING OF THE GROWTH-SUPPRESSINGHUMAN HOMOLOG OF THE MURINE GAS1 GENE, Proceedings of the National Academy of Sciences of the United Statesof America, 91(5), 1994, pp. 1848-1852
We describe the isolation, growth-suppressing activity, and chromosoma
l localization of the human homologue of the murine growth-arrest-spec
ific gene gas1. Overexpression of h-gas1 is able to block cell prolife
ration in the A549 lung carcinoma and the T24 bladder carcinoma cell l
ines. No effect was observed when h-gas1 was introduced into the osteo
sarcoma cell line SAOS-2 and into the adenovirus-type-5 transformed ce
ll line 293. This finding is related to our previous evidence that sim
ian virus 40-transformed NIH 3T3 cells are also refractory to murine g
as1 overexpression, suggesting that the retinoblastoma and/or p53 gene
products have an active role in mediating the growth-suppressing effe
ct of gas1. We also show that h-gas1 is on chromosome 9q21.3-22.1, in
a region considered to be a fragile site. Altogether, the results rais
e the possibility that h-gas1 may be a target for genetic alterations
leading to its inactivation in tumor cells.