CAPILLARY ZONE ELECTROPHORESIS SEPARATION OF KININS USING A NOVEL LASER FLUORESCENCE DETECTOR

Authors
KIST TBL TERMIGNONI C GRIENEISEN HPH
Citation
Tbl. Kist et al., CAPILLARY ZONE ELECTROPHORESIS SEPARATION OF KININS USING A NOVEL LASER FLUORESCENCE DETECTOR, Brazilian journal of medical and biological research, 27(1), 1994, pp. 11-19
Citations number
25
Categorie Soggetti
Medicine, Research & Experimental
Journal title
Brazilian journal of medical and biological research → ACNP
ISSN journal
0100879X
Volume
27
Issue
1
Year of publication
1994
Pages
11 - 19
Database
ISI
SICI code
0100-879X(1994)27:1<11:CZESOK>2.0.ZU;2-Y
Abstract
1. Bradykinin, Lys-bradykinin, Met-Lys-bradykinin, des-Arg(9)-bradykin in and des-Arg(1)-bradykinin were separated by capillary zone electrop horesis in an apparatus constructed in our laboratory which utilizes a novel N-2 pulsed laser-induced fluorescence detector. 2. Detection li mits of 1.2 fmol for fluorescamine-derivatized bradykinin and 90 attom ol for O-phthaldiadehyde-derivatized bradykinin were achieved. 3. This powerful analytical tool is described and its successful application to the measurements of bradykinin after enzymatic release from blood i s documented.