IDENTIFICATION OF AMINO-ACID BASE CONTACTS IN THE MYC DNA COMPLEX BY SITE-SPECIFIC BROMOURACIL MEDIATED PHOTO-CROSS-LINKING

Authors
DONG QP BLATTER EE EBRIGHT YW BISTER K EBRIGHT RH
Citation
Qp. Dong et al., IDENTIFICATION OF AMINO-ACID BASE CONTACTS IN THE MYC DNA COMPLEX BY SITE-SPECIFIC BROMOURACIL MEDIATED PHOTO-CROSS-LINKING, EMBO journal, 13(1), 1994, pp. 200-204
Citations number
32
Categorie Soggetti
Biology
Journal title
EMBO journal → ACNP
ISSN journal
02614189
Volume
13
Issue
1
Year of publication
1994
Pages
200 - 204
Database
ISI
SICI code
0261-4189(1994)13:1<200:IOABCI>2.0.ZU;2-6
Abstract
Myc binds to a 6 bp 2-fold symmetric DNA site: 5'-C(-3)A(-2)C(-1)G(+1) T(+2)G(+3)-3'. Using site-specific 5-bromouracil mediated photocrossli nking, we show that His336 of Myc contacts, or is close to, the thymin e 5-methyl group at 2-fold symmetry-related positions -2 and +2 of the DNA site in the Myc- DNA complex. Our results strongly suggest that h omologous amino acids of Myc and Max make equivalent contacts in the r espective protein-DNA complexes.